Frontiers in fluorescence microscopy

Authors: José Rino, José Braga, Ricardo Henriques, Maria Carmo-Fonseca
Review published in International Journal of Developmental Biology, January 2009
Publisher: UPV/EHU Press

Frontiers in fluorescence microscopy
DOI: 10.1387/ijdb.072351jr

The manuscript published in the International Journal of Developmental Biology by José Rino and colleagues provides a historical perspective on the development of microscopy, focusing on light microscopy and its impact on the study of organisms and cells. The authors discuss the early history of microscopy, the theoretical limit of resolution, and the invention of confocal and multi-photon microscopy. They also cover the history of fluorescence microscopy, its limitations, and the development of new techniques to overcome them. The manuscript emphasizes the importance of fluorescence microscopy in modern biology and its potential for future discoveries. The authors describe various fluorescence microscopy techniques, including FRAP, FLIP, iFRAP, photoactivation, FCS, and the use of super-lenses. Additionally, the manuscript mentions specific studies and advancements in fluorescence microscopy, such as quantitative FRET measurements, fluorescence correlation spectroscopy, photoactivatable fluorescent proteins, and the development of I5M, a system with better than 100 nm axial resolution. Overall, the manuscript highlights the significant role of microscopy, particularly fluorescence microscopy, in advancing our understanding of the biological world.