PALM and STORM - unlocking live‐cell super‐resolution

Authors: Ricardo Henriques, Caron Griffiths, E Hesper Rego, Musa M Mhlanga
Technologies: QuickPALM
Review published in Biopolymers, January 2011
Publisher: Wiley Subscription Services, Inc., A Wiley Company Hoboken

PALM and STORM - unlocking live‐cell super‐resolution
DOI: 10.1002/bip.21586

The manuscript "PALM and STORM: Unlocking Live-Cell Super-Resolution" by Ricardo Henriques et al. discusses recent advances in single molecule localization microscopy (SMLM) for achieving super-resolution imaging in living cells. The authors examine techniques such as photo-activated localization microscopy (PALM), fluorescence PALM (FPALM), reconstruction microscopy (STORM), direct STORM (dSTORM), and PALM with independent running acquisition (PALMIRA), which have shown sub-nanometer resolution but are hampered by their inability to be robustly used for live-cell imaging. The authors also discuss strategies to address the challenges of achieving 'EM-like' resolution while preserving the advantages of live-cell fluorescence microscopy, including innovations in imaging, microscopy, and sample preparation (hardware) and computational techniques (software).