Single-molecule localization super-resolution microscopy - deeper and faster
Technologies: QuickPALM
Review published in Microscopy and microanalysis, January 2012
Publisher: Oxford University Press
The manuscript "Single-Molecule Localization Super-Resolution Microscopy: Deeper and Faster" by Sébastien Herbert et al. (2012) provides an in-depth review of single-molecule localization microscopy (SMLM), a family of super-resolution methods that achieve molecular localization accuracies at the nanometer level. The manuscript discusses the history and development of SMLM, its applications in studying structures and interactions not easily differentiated with classical optical microscopes, and its potential future applications. The authors also mention the challenges of live-cell imaging in SMLM and the importance of high molecular localization densities, sensitivity of imaging hardware, algorithmic capacity, and fluorophore photon output and contrast ratios.