PALM and STORM - Into large fields and high-throughput microscopy with sCMOS detectors

Authors: Pedro Almada, Siân Culley, Ricardo Henriques
Technologies: QuickPALM
Review published in Methods, January 2015
Publisher: Academic Press
PALM and STORM - Into large fields and high-throughput microscopy with sCMOS detectors
DOI: 10.1016/j.ymeth.2015.06.004

The manuscript by Almada, Culley, and Henriques (Methods, 2015) explores the use of Single Molecule Localization Microscopy (SMLM) techniques, specifically Photo-Activation Localization Microscopy (PALM) and Stochastic Optical Reconstruction Microscopy (STORM), for achieving super-resolution in fluorescence microscopy. The authors discuss the advantages of sCMOS cameras for SMLM, including their large field of view and short exposure times, and provide guidance on excitation path, laser launching, and large FOV illumination considerations for designing a high-throughput SMLM system.