Research Team
Siân was a 8-year Postdoc (now PI) in our research group. Worked on 17 publications. Contributed to 7 technologies. Collaborated with 13 labs.
My research centres on developing novel analytical approaches for fluorescence microscopy. I helped develop the Super-Resolution Radial Fluctuations (SRRF) algorithm, which extracts super-resolution information from conventional fluorescence microscopy time-series, and allows for live-cell super-resolution imaging in physiological conditions without specialised fluorophores. I also developed the Super-Resolution Quantitative Image Rating and Reporting of Error Locations (SQUIRREL) algorithm, which is a method for assessing the quality of super-resolution microscopy images. I also develop analytical methods for microbiology, including the creation of image analysis pipelines for characterising nuclear envelope architecture during mitosis in S. pombe (in collaboration with Gautam Dey, LMCB) and characterising cell division in thermophilic Archaea (in collaboration with Uwe Schmidt, MPI-CBG).
In the news
- 2023-11-27: News outlet Photonics.com highlights Laine et al. Nature Methods 2023
- 2023-11-20: News outlet The Science Times highlights Laine et al. Nature Methods 2023
- 2023-11-16: News outlet Optics.org highlights Laine et al. Nature Methods 2023
- 2023-11-14: Blog Springer Nature Protocols and Methods Community highlights Laine et al. Nature Methods 2023
- 2023-11-14: News outlet Phys.org highlights Laine et al. Nature Methods 2023
- 2021-07-14: News outlet Technology Networks highlights Weigert et al. Nature methods 2018
- 2020-09-12: News outlet NRK highlights Risa et al. Science 2020
- 2020-09-02: News outlet Nature Asia highlights Dey et al. Nature 2020
- 2020-08-31: News outlet Azom.com highlights Gustafsson et al. Nature communications 2016
- 2020-08-28: News outlet Scitech Daily highlights Risa et al. Science 2020
- 2020-08-12: News outlet NCYT - Noticias de la Ciencia y la Technologia highlights Risa et al. Science 2020
- 2020-08-06: News outlet UPI.com highlights Risa et al. Science 2020
- 2018-12-10: News outlet VBIO highlights Weigert et al. Nature methods 2018
- 2018-12-07: News outlet Innovations Report highlights Weigert et al. Nature methods 2018
- 2018-12-07: News outlet Informationsdienst Wissenschaft highlights Weigert et al. Nature methods 2018
- 2018-05-14: News outlet physicsworld.com highlights Culley et al. Nature methods 2018
Collaborations
Anđela Šarić
IST
Vienna, Austria
Joint:
2
Christophe Leterrier
Aix Marseille Université, CNRS, INP
Marseille, France
Joint:
10
Loïc Royer
Chan Zuckerberg Biohub
San Francisco, USA
Joint:
2
Serge Mostowy
School of Hygiene & Tropical Medicine
London, UK
Joint:
2
Technologies
Publications with our group (see more on Google Scholar)
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High-fidelity 3D live-cell nanoscopy through data-driven enhanced super-resolution radial fluctuation Romain F. Laine, Hannah S. Heil, Simao Coelho, Jonathon Nixon-Abell, Angélique Jimenez, Theresa Wiesner, Damián Martínez, Tommaso Galgani, Louise Régnier, Aki Stubb, Gautier Follain, Samantha Webster, Jesse Goyette, Aurelien Dauphin, Audrey Salles, Siân Culley, Guillaume Jacquemet, Bassam Hajj, Christophe Leterrier, Ricardo Henriques Paper published in Nature Methods, January 2023 Technologies: NanoJ, NanoJ-eSRRF, NanoJ-SQUIRREL, NanoJ-SRRF, NanoPyx and Nuclear-Pores as references Funded by: CZI, EMBO, ERC, FCT, H2021, H2022, InnOValley and Wellcome Trust News: Photonics.com, The Science Times, Optics.org and Phys.org Blogs: Springer Nature Protocols and Methods Community DOI: 10.1038/s41592-023-02057-w |
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Physical mechanisms of ESCRT-III–driven cell division Lena Harker-Kirschneck, Anne E. Hafner, Tina Yao, Christian Vanhille-Campos, Xiuyun Jiang, Andre Pulschen, Fredrik Hurtig, Dawid Hryniuk, Siân Culley, Ricardo Henriques, Buzz Baum, Anđela Šarić Paper published in Proceedings of the National Academy of Sciences, January 2022 Funded by: Wellcome Trust DOI: 10.1073/pnas.2107763119 |
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An Introduction to Live-Cell Super-Resolution Imaging Siân Culley, Pedro Matos Pereira, Romain F Laine, Ricardo Henriques Book chapter published in Imaging from Cells to Animals In Vivo, January 2020 Technologies: CARE, NanoJ, NanoJ-Fluidics, NanoJ-SQUIRREL and QuickPALM DOI: 10.1201/9781315174662-4 |
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Closed mitosis requires local disassembly of the nuclear envelope Gautam Dey, Siân Culley, Scott Curran, Uwe Schmidt, Ricardo Henriques, Wanda Kukulski, Buzz Baum Paper published in Nature, January 2020 Technologies: CARE, NanoJ, NanoJ-SRRF and Nuclear-Pores as references Funded by: BBSRC and Wellcome Trust News: Nature Asia DOI: 10.1038/s41586-020-2648-3 |
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Live imaging of a hyperthermophilic archaeon reveals distinct roles for two ESCRT-III homologs in ensuring a robust and symmetric division Andre Arashiro Pulschen, Delyan R Mutavchiev, Siân Culley, Kim Nadine Sebastian, Jacques Roubinet, Marc Roubinet, Gabriel Tarrason Risa, Marleen van Wolferen, Chantal Roubinet, Uwe Schmidt, Gautam Dey, Sonja-Verena Albers, Ricardo Henriques, Buzz Baum Paper published in Current Biology, January 2020 Technologies: Sulfoscope Funded by: Wellcome Trust DOI: 10.1016/j.cub.2020.05.021 |
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The proteasome controls ESCRT-III–mediated cell division in an archaeon Gabriel Tarrason Risa, Fredrik Hurtig, Sian Bray, Anne E. Hafner, Lena Harker-Kirschneck, Peter Faull, Colin Davis, Dimitra Papatziamou, Delyan R. Mutavchiev, Catherine Fan, Leticia Meneguello, Andre Arashiro Pulschen, Gautam Dey, Siân Culley, Mairi Kilkenny, Diorge P. Souza, Luca Pellegrini, Robertus A. M. de Bruin, Ricardo Henriques, Ambrosius P. Snijders, Anđela Šarić, Ann-Christin Lindås, Nicholas P. Robinson, Buzz Baum Paper published in Science, January 2020 Technologies: NanoJ News: NRK, Scitech Daily, NCYT - Noticias de la Ciencia y la Technologia and UPI.com DOI: 10.1126/science.aaz2532 |
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Between life and death - strategies to reduce phototoxicity in super-resolution microscopy Kalina L Tosheva, Yue Yuan, Pedro Matos Pereira, Siân Culley, Ricardo Henriques Review published in Journal of Physics D - Applied Physics, January 2020 Technologies: CARE, NanoJ and NanoJ-Fluidics Funded by: BBSRC and Wellcome Trust DOI: 10.1088/1361-6463/ab6b95 |
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NanoJ - a high-performance open-source super-resolution microscopy toolbox Romain F Laine, Kalina L Tosheva, Nils Gustafsson, Robert D M Gray, Pedro Almada, David Albrecht, Gabriel T Risa, Fredrik Hurtig, Ann-Christin Lindås, Buzz Baum, Jason Mercer, Christophe Leterrier, Pedro M Pereira, Siân Culley, Ricardo Henriques Paper published in Journal of physics D - Applied physics, January 2019 Technologies: CARE, NanoJ, NanoJ-Fluidics, NanoJ-SQUIRREL, NanoJ-SRRF, NanoJ-VirusMapper and QuickPALM Funded by: BBSRC and Wellcome Trust DOI: 10.1088/1361-6463/ab0261 |
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Fix your membrane receptor imaging - actin cytoskeleton and CD4 membrane organization disruption by chemical fixation Pedro M Pereira, David Albrecht, Siân Culley, Caron Jacobs, Mark Marsh, Jason Mercer, Ricardo Henriques Published in Frontiers in immunology, January 2019 Technologies: NanoJ, NanoJ-Fluidics and NanoJ-SQUIRREL Funded by: BBSRC and Wellcome Trust DOI: 10.3389/fimmu.2019.00675 |
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Quantitative mapping and minimization of super-resolution optical imaging artifacts Siân Culley, David Albrecht, Caron Jacobs, Pedro Matos Pereira, Christophe Leterrier, Jason Mercer, Ricardo Henriques Paper published in Nature methods, January 2018 Technologies: NanoJ, NanoJ-SQUIRREL and QuickPALM Funded by: BBSRC and Wellcome Trust News: physicsworld.com DOI: 10.1038/nmeth.4605 |
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SRRF - Universal live-cell super-resolution microscopy Siân Culley, Kalina L Tosheva, Pedro Matos Pereira, Ricardo Henriques Paper published in The international journal of biochemistry & cell biology, January 2018 Technologies: NanoJ, NanoJ-SQUIRREL and NanoJ-SRRF Funded by: BBSRC and Wellcome Trust DOI: 10.1016/j.biocel.2018.05.014 |
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Content-aware image restoration - pushing the limits of fluorescence microscopy Martin Weigert, Uwe Schmidt, Tobias Boothe, Andreas Müller, Alexandr Dibrov, Akanksha Jain, Benjamin Wilhelm, Deborah Schmidt, Coleman Broaddus, Siân Culley, Mauricio Rocha-Martins, Fabián Segovia-Miranda, Caren Norden, Ricardo Henriques, Marino Zerial, Michele Solimena, Jochen Rink, Pavel Tomancak, Loic Royer, Florian Jug, Eugene W. Myers Paper published in Nature methods, January 2018 Technologies: CARE and NanoJ-SQUIRREL Funded by: BBSRC and Wellcome Trust News: Technology Networks, VBIO, Innovations Report and Informationsdienst Wissenschaft DOI: 10.1038/s41592-018-0216-7 |
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Heterogeneous localisation of membrane proteins in Staphylococcus aureus Felix Weihs, Katarzyna Wacnik, Robert D. Turner, Siân Culley, Ricardo Henriques, Simon J. Foster Paper published in Scientific Reports, January 2018 Technologies: NanoJ and NanoJ-SRRF Funded by: BBSRC and Wellcome Trust DOI: 10.1038/s41598-018-21750-x |
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Infection counter - automated quantification of in vitro virus replication by fluorescence microscopy Siân Culley, Greg Towers, David Selwood, Ricardo Henriques, Joe Grove Paper published in Viruses, January 2016 Technologies: Infection-Counter Funded by: BBSRC DOI: 10.3390/v8070201 |
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Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations Nils Gustafsson, Siân Culley, George Ashdown, Dylan M. Owen, Pedro Matos Pereira, Ricardo Henriques Paper published in Nature communications, January 2016 Technologies: NanoJ-SRRF and QuickPALM Funded by: BBSRC News: Azom.com DOI: 10.1038/ncomms12471 |
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Mitochondria mediate septin cage assembly to promote autophagy of Shigella Andrea Sirianni, Sina Krokowski, Damián Lobato‐Márquez, Stephen Buranyi, Julia Pfanzelter, Dieter Galea, Alexandra Willis, Siân Culley, Ricardo Henriques, Gerald Larrouy‐Maumus, Michael Hollinshead, Vanessa Sancho‐Shimizu, Michael Way, Serge Mostowy Paper published in EMBO reports, January 2016 Funded by: BBSRC DOI: 10.15252/embr.201541832 |
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PALM and STORM - Into large fields and high-throughput microscopy with sCMOS detectors Pedro Almada, Siân Culley, Ricardo Henriques Review published in Methods, January 2015 Technologies: QuickPALM DOI: 10.1016/j.ymeth.2015.06.004 |
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