Mapping the early stages of HIV-1 infection by live-cell 4D Super-Resolution Microscopy

Funding Image
Agency: EMBO
Type: Postdoctoral Fellowships
Principal Investigator: Hannah Heil
Start-date: September 2021
End-date: August 2023

The project focused on investigating the early stages of HIV-1 infection using advanced imaging technology, including machine-learning, microfluidics, and super-resolution imaging. The project aims to provide new insights into virus-host interactions and develop transferable technologies for the research community.

Technology explored

NanoJ-Fluidics NanoJ-Fluidics
NanoJ-VirusMapper NanoJ-VirusMapper
Nuclear-Pores as references Nuclear-Pores as references

Supported publications

High-fidelity 3D live-cell nanoscopy through data-driven enhanced super-resolution radial fluctuation
Romain F. Laine, Hannah S. Heil, Simao Coelho, Jonathon Nixon-Abell, Angélique Jimenez, Theresa Wiesner, Damián Martínez, Tommaso Galgani, Louise Régnier, Aki Stubb, Gautier Follain, Samantha Webster, Jesse Goyette, Aurelien Dauphin, Audrey Salles, Siân Culley, Guillaume Jacquemet, Bassam Hajj, Christophe Leterrier, Ricardo Henriques
Paper published in Nature Methods, November 2023
Technologies: CARE, NanoJ, NanoJ-eSRRF, NanoJ-SQUIRREL, NanoJ-SRRF, NanoPyx and Nuclear-Pores as references
Funded by: CZI, EMBO, ERC, FCT, H2021, H2022, InnOValley and Wellcome Trust
News:, The Science Times, and
Blogs: Springer Nature Protocols and Methods Community
DOI: 10.1038/s41592-023-02057-w
Mapping molecular complexes with super-resolution microscopy and single-particle analysis
Afonso Mendes, Hannah S. Heil, Simao Coelho, Christophe Leterrier, Ricardo Henriques
Review published in Open Biology, July 2022
Technologies: NanoJ-VirusMapper, Nuclear-Pores as references and ZeroCostDL4Mic
Funded by: EMBO, ERC and Wellcome Trust
DOI: 10.1098/rsob.220079
Trimethine cyanine dyes as NA-sensitive probes for visualization of cell compartments in fluorescence microscopy
Daria Aristova, Roman Selin, Hannah Sophie Heil, Viktoriia Kosach, Yuriy Slominsky, Sergiy Yarmoluk, Vasyl Pekhnyo, Vladyslava Kovalska, Ricardo Henriques, Andriy Mokhir
Paper published in ACS omega, January 2022
Funded by: EMBO, ERC and H2021
DOI: 10.1021/acsomega.2c05231
Expansion Microscopy on Saccharomyces cerevisiae
AG Korovesi, L Morgado, M Fumasoni, R Henriques, HS Heil, M Del Rosario
Paper published in microPublication Biology, January 2022
Technologies: CARE
Funded by: EMBO, ERC and Wellcome Trust
DOI: 10.17912/micropub.biology.000566