Fix your membrane receptor imaging - actin cytoskeleton and CD4 membrane organization disruption by chemical fixation


Authors: Pedro M Pereira, David Albrecht, Siân Culley, Caron Jacobs, Mark Marsh, Jason Mercer, Ricardo Henriques
Technologies: CARE, NanoJ, NanoJ-Fluidics and NanoJ-SQUIRREL
Published in Frontiers in immunology, January 2019
Publisher: Frontiers

Fix your membrane receptor imaging - actin cytoskeleton and CD4 membrane organization disruption by chemical fixation
DOI: 10.3389/fimmu.2019.00675

The manuscript "Fix Your Membrane Receptor Imaging: Actin Cytoskeleton and CD4 Membrane Organization Disruption by Chemical Fixation," published in Frontiers in Immunology, explores the impact of various chemical fixation methods on membrane protein organization, specifically CD4, using single-molecule localization microscopy (SMLM). The authors discovered that suboptimal fixation procedures, which disrupt the actin cytoskeleton, result in alterations in CD4 membrane organization. They also demonstrated that the fixation protocol influences CD4 cluster size and density at the cell surface. The study underscores the significance of meticulous sample preparation and handling throughout all stages leading to the final image for accurate and dependable results in super-resolution microscopy.