Research Team
Christoph was a 1-year Postdoc (now PI) in our research group. Worked on 3 publications. Contributed to 2 technologies. Collaborated with 9 labs.
My past: I studied biology in the beautiful and romantic city Würzburg in Germany. Afterwards, I moved to Frankfurt to do my PhD in Mike Heilemann research group, investigating nucleoid architecture in E. coli cells using single-molecule localization microscopy (SMLM), a super-resolution approach that provides near molecular resolution. After figuring out how to directly label the nucleoid and how to combine different super-resolution methods (which took some time), my work resulted in several imaging approaches that allow for three-color SMLM in individual cells at spatial resolutions of ~ 20 – 40 nm. This is exactly what we require to explore the inner life of bugs at the nanoscale and understand how different processes and stimuli affect bacterial homeostasis and nucleoid organization.
My present: With the developed toolbox, I investigate how E. coli cells replicate and segregate their chromosome and how biological processes such as transcription and translation shape the nucleoid in a dynamic manner. As most of my work was performed in fixed specimen, I am very happy to join the Henriques group as an EMBO short term fellow, to perform live-cell imaging and ultimately provide a dynamic, super-resolved picture of bacterial chromosome replication.
My future: I want to use super-resolution microscopy to gain a deep understanding of how antimicrobial compounds perturb microbial cell homeostasis. This knowledge will become extremely important for fighting antimicrobial resistance and designing new drugs.
In the news
- 2021-11-03: Editorial in Nature Methods highlights Chamier et al. Nature communications 2021
- 2021-05-07: News outlet Expresso highlights Chamier et al. Nature communications 2021
- 2021-04-26: News outlet AZO Life Sciences highlights Chamier et al. Nature communications 2021
- 2021-04-26: News outlet Drug Target Review highlights Chamier et al. Nature communications 2021
- 2021-04-24: News outlet Nanotechnology Now highlights Chamier et al. Nature communications 2021
- 2021-04-24: News outlet The Medical News highlights Chamier et al. Nature communications 2021
- 2021-04-16: Blog Microbiome Digest - Bik's Picks highlights Chamier et al. Nature communications 2021
- 2020-04-21: Editorial in Nature highlights Chamier et al. Nature communications 2021
Collaborations
Christophe Leterrier
Aix Marseille Université, CNRS, INP
Marseille, France
Joint:
10
Loïc Royer
Chan Zuckerberg Biohub
San Francisco, USA
Joint:
2
Séamus Holden
Warwick University
Warwick, UK
Joint:
5
Yoav Shechtman
Technion - Israel Institute of Technology
Haifa, Israel
Joint:
4
Technologies
Publications with our group (see more on Google Scholar)
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Transertion and cell geometry organize the Escherichia coli nucleoid during rapid growth Christoph Spahn, Stuart Middlemiss, Estibaliz Gómez-de-Mariscal, Ricardo Henriques, Helge B. Bode, Séamus Holden, Mike Heilemann Preprint published in bioRxiv, January 2023 Technologies: CARE, DeepAutoFocus, DeepBacs and ZeroCostDL4Mic Funded by: EMBO, ERC, H2021 and H2022 DOI: 10.1101/2023.10.16.562172 |
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DeepBacs for multi-task bacterial image analysis using open-source deep learning approaches Christoph Spahn, Estibaliz Gómez-de-Mariscal, Romain F. Laine, Pedro M. Pereira, Lucas von Chamier, Mia Conduit, Mariana G. Pinho, Guillaume Jacquemet, Séamus Holden, Mike Heilemann, Ricardo Henriques Paper published in Communications Biology, January 2022 Technologies: BioImage Model Zoo, CARE, DeepBacs, NanoJ, NanoJ-SQUIRREL and ZeroCostDL4Mic Funded by: CZI, ERC, FCT and Wellcome Trust DOI: 10.1038/s42003-022-03634-z |
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Democratising deep learning for microscopy with ZeroCostDL4Mic Lucas von Chamier, Romain F. Laine, Johanna Jukkala, Christoph Spahn, Daniel Krentzel, Elias Nehme, Martina Lerche, Sara Hernández-Pérez, Pieta K. Mattila, Eleni Karinou, Séamus Holden, Ahmet Can Solak, Alexander Krull, Tim-Oliver Buchholz, Martin L. Jones, Loïc A. Royer, Christophe Leterrier, Yoav Shechtman, Florian Jug, Mike Heilemann, Guillaume Jacquemet, Ricardo Henriques Paper published in Nature communications, January 2021 Technologies: CARE, NanoJ, NanoJ-SQUIRREL, NanoJ-SRRF and ZeroCostDL4Mic Funded by: EMBO, ERC and Wellcome Trust News: AZO Life Sciences, Drug Target Review, Nanotechnology Now and The Medical News Blogs: Microbiome Digest - Bik's Picks DOI: 10.1038/s41467-021-22518-0 |
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