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DL4MicEverywhere


GitHub:
- HenriquesLab/DL4MicEverywhere

Publication: Hidalgo-Cenalmor et al. Nature Methods 2024
- 8

DL4MicEverywhere is an open-source platform that aims to make deep learning more accessible for bioimage analysis. It builds on ZeroCostDL4Mic, extending it so that models can be trained and run across a wider range of computing environments.

The key goal of DL4MicEverywhere is to democratize deep learning for microscopy image analysis by tackling some of the main barriers to adoption. Many researchers have no access to training datasets large enough, or annotated well enough, to produce an accurate model. Training such networks also needs GPUs, which are expensive. Few biomedical researchers have experience designing, training and deploying them.

DL4MicEverywhere packages deep learning methods for bioimage analysis into Docker containers. Training and inference then run on laptops, workstations, clusters and cloud platforms with no dependencies to install. Pre-trained models are shared so that work can start on real data straight away.

A key emphasis in DL4MicEverywhere is on transparency, reproducibility and convenience. Self-contained Docker images behave the same on every operating system, and the documentation includes usage tutorials. Because the code is open, the methods can be reviewed, improved and extended by anyone. Researchers are encouraged to containerise their own models and contribute them back.


Publications featuring DL4MicEverywhere

Packaging Jupyter notebooks as installable desktop apps using LabConstrictor
Iván Hidalgo-Cenalmor, Marcela Xiomara Rivera Pineda, Bruno M Saraiva, Ricardo Henriques, Guillaume Jacquemet
Preprint published in arXiv, March 2026
Technologies: CARE (), CellTracksColab (), DL4MicEverywhere (), mAIcrobe () and ZeroCostDL4Mic ()
Funded by: Chan Zuckerberg Initiative (CZI), The Kavli Foundation, and The Wellcome Trust, EMBO, ERC, H2022 and La Caixa Foundation
DOI: 10.48550/arXiv.2603.10704
EZInput: A Cross-Environment Python Library for Easy UI Generation in Scientific Computing
Bruno M Saraiva, Iván Hidalgo-Cenalmor, António D Brito, Damián Martínez, Tayla Shakespeare, Guillaume Jacquemet, Ricardo Henriques
Preprint published in arXiv, January 2026
Technologies: DL4MicEverywhere (), mAIcrobe (), NanoJ-eSRRF (), NanoPyx () and ZeroCostDL4Mic ()
Funded by: Chan Zuckerberg Initiative (CZI), The Kavli Foundation, and The Wellcome Trust, CZI, EMBO, ERC, H2021 and H2022
DOI: 10.48550/arXiv.2601.08859
Rxiv-Maker: An Automated Template Engine for Streamlined Scientific Publications
Bruno M Saraiva, Guillaume Jaquemet, Ricardo Henriques
Preprint published in arXiv, June 2025
Technologies: DL4MicEverywhere ()
Funded by: Chan Zuckerberg Initiative (CZI), The Kavli Foundation, and The Wellcome Trust, CZI, EMBO, ERC, H2021 and H2022
DOI: 10.48550/arXiv.2508.00836
ReScale4DL: Balancing Pixel and Contextual Information for Enhanced Bioimage Segmentation
Mariana G. Ferreira, Bruno M. Saraiva, António D. Brito, Mariana G. Pinho, Ricardo Henriques, Estibaliz Gómez-de-Mariscal
Preprint published in bioRxiv, April 2025
Technologies: BioImage Model Zoo (), DeepBacs (), DL4MicEverywhere (), NanoPyx (), Rescale4DL () and ZeroCostDL4Mic ()
Funded by: CZI, EMBO, ERC, H2021 and H2022
DOI: 10.1101/2025.04.09.647871
DL4MicEverywhere: deep learning for microscopy made flexible, shareable and reproducible
Iván Hidalgo-Cenalmor, Joanna W. Pylvänäinen, Mariana G. Ferreira, Craig T. Russell, Alon Saguy, Ignacio Arganda-Carreras, Yoav Shechtman, Guillaume Jacquemet, Ricardo Henriques, Estibaliz Gómez-de-Mariscal
Paper published in Nature Methods, May 2024
Technologies: BioImage Model Zoo (), DL4MicEverywhere () and ZeroCostDL4Mic ()
Funded by: EMBO, ERC, H2021 and H2022
DOI: 10.1038/s41592-024-02295-6

Funding contributing to DL4MicEverywhere

VirusAwareScopes: Machine Learning-Driven Adaptive Microscopy for Long-Term Viral Infection Studies
Ricardo Henriques
Alias: VirusAwareScopes
Funded by: La Caixa Foundation - Health Research
Duration: November 2025 - October 2028
Publications: 2
Democratizing Deep Learning for Microscopists with DL4MicEverywhere
Ricardo Henriques, Guillaume Jacquemet, Caron Jacobs
Funded by: Chan Zuckerberg Initiative (CZI), The Kavli Foundation, and The Wellcome Trust - Essential Open Source Software for Science (Cycle 6)
Duration: August 2025 - July 2027
Publications: 10