Democratizing Deep Learning for Microscopists with DL4MicEverywhere


Funding Image
Agency: Chan Zuckerberg Initiative (CZI), The Kavli Foundation, and The Wellcome Trust
Type: Essential Open Source Software for Science (Cycle 6)
Principal Investigator: Ricardo Henriques
Investigators: Ricardo Henriques, Guillaume Jacquemet, Caron Jacobs
Start-date: August 2025
End-date: July 2027
Grant Code: 313383/Z/24/Z; EOSS6-0000000260

Technology explored


DL4MicEverywhere DL4MicEverywhere Newest update: 2025-11-19

Supported publications


EZInput - A Cross-Environment Python Library for Easy UI Generation in Scientific Computing
Bruno M Saraiva, Iván Hidalgo-Cenalmor, António D Brito, Damián Martínez, Tayla Shakespeare, Guillaume Jacquemet, Ricardo Henriques
Preprint published in arXiv, January 2026
Technologies: EZInput () and NanoPyx ()
Funded by: Chan Zuckerberg Initiative (CZI), The Kavli Foundation, and The Wellcome Trust, CZI, EMBO and ERC
DOI: 10.48550/arXiv.2601.08859
PhotoFiTT - a quantitative framework for assessing phototoxicity in live-cell microscopy experiments
Mario Del Rosario, Estibaliz Gómez-de-Mariscal, Leonor Morgado, Raquel Portela, Guillaume Jacquemet, Pedro M. Pereira, Ricardo Henriques
Paper published in Nature Communications, December 2025
Technologies: mAIcrobe (), PhotoFiTT () and ZeroCostDL4Mic ()
Funded by: Chan Zuckerberg Initiative (CZI), The Kavli Foundation, and The Wellcome Trust, CZI, EMBO, ERC, FCT, H2021 and H2022
DOI: 10.1038/s41467-025-66209-6
Expansion and fluctuations-enhanced microscopy for nanoscale molecular profiling of cells and tissues
Dominik Kylies, Hannah S. Heil, Arturo G. Vesga, Mario Del Rosario, Maria Schwerk, Malte Kuehl, Milagros N. Wong, Victor G. Puelles, Ricardo Henriques
Paper published in Nature Protocols, July 2025
Technologies: NanoJ (), NanoJ-eSRRF (), NanoJ-SQUIRREL (), NanoJ-SRRF (), NanoPyx () and Nuclear-Pores as references
Funded by: Chan Zuckerberg Initiative (CZI), The Kavli Foundation, and The Wellcome Trust, CZI, EMBO, ERC, FCT, H2021 and H2022
DOI: 10.1038/s41596-025-01178-0
Structural Repetition Detector for multi-scale quantitative mapping of molecular complexes through microscopy
Afonso Mendes, Bruno M. Saraiva, Guillaume Jacquemet, João I. Mamede, Christophe Leterrier, Ricardo Henriques
Paper published in Nature Communications, July 2025
Technologies: Nuclear-Pores as references and SReD ()
Funded by: Chan Zuckerberg Initiative (CZI), The Kavli Foundation, and The Wellcome Trust, CZI, EMBO, ERC, H2021 and H2022
DOI: 10.1038/s41467-025-60709-1
Rxiv-Maker - An Automated Template Engine for Streamlined Scientific Publications
Bruno M Saraiva, Guillaume Jaquemet, Ricardo Henriques
Preprint published in arXiv, June 2025
Technologies: DL4MicEverywhere ()
Funded by: Chan Zuckerberg Initiative (CZI), The Kavli Foundation, and The Wellcome Trust, CZI, EMBO, ERC, H2021 and H2022
DOI: 10.48550/arXiv.2508.00836
Nanoscale imaging of biological systems via expansion and super-resolution microscopy
Daria Aristova, Dominik Kylies, Mario Del Rosario, Hannah S. Heil, Maria Schwerk, Malte Kuehl, Milagros N. Wong, Ricardo Henriques, Victor G. Puelles
Paper published in Applied Physics Reviews, April 2025
Technologies: NanoJ-eSRRF () and NanoJ-SRRF ()
Funded by: Chan Zuckerberg Initiative (CZI), The Kavli Foundation, and The Wellcome Trust, CZI, EMBO, ERC, FCT, H2021 and H2022
DOI: 10.1063/5.0240464
This Microtubule Does Not Exist - Super‐Resolution Microscopy Image Generation by a Diffusion Model
Alon Saguy, Tav Nahimov, Maia Lehrman, Estibaliz Gómez‐de‐Mariscal, Iván Hidalgo‐Cenalmor, Onit Alalouf, Ashwin Balakrishnan, Mike Heilemann, Ricardo Henriques, Yoav Shechtman
Paper published in Small Methods, January 2025
Technologies: CARE () and ZeroCostDL4Mic ()
Funded by: Chan Zuckerberg Initiative (CZI), The Kavli Foundation, and The Wellcome Trust, CZI, EMBO, ERC, H2021 and H2022
DOI: 10.1002/smtd.202400672
Non-synaptic exocytosis along the axon shaft and its regulation by the submembrane periodic skeleton
Theresa Wiesner, Christopher Louis Parperis, Fanny Boroni-Rueda, Nicolas Jullien, Afonso Mendes, Léa Marie, Louisa Mezache, Marie-Jeanne Papandréou, Ricardo Henriques, Christophe Leterrier
Preprint published in bioRxiv, January 2025
Technologies: CARE () and SReD ()
Funded by: Chan Zuckerberg Initiative (CZI), The Kavli Foundation, and The Wellcome Trust, EMBO and ERC
DOI: 10.1101/2025.09.17.676728