Cutting-edge super-resolution image analysis in napari through NanoJ

Agency: CZI
Type: Applications - napari Plugin Foundations grants
Principal Investigator: Ricardo Henriques
Investigators: Bruno Saraiva, Ricardo Henriques
Start-date: January 2023
End-date: December 2023
Website: https://www.napari-hub.org/plugins/napari-nanopyx

NanoJ is an instrumental ImageJ-based computational framework that provides several analytical tasks to enhance or enable super-resolution microscopy. It also hosts popular GPU-based algorithms, including NanoJ-SRRF, which provides the capacity to super-resolve images from conventional microscopes without needing super-resolution specific hardware; and NanoJ-SQUIRREL, which quantitatively detects artifacts created by super-resolution methods. While the use of NanoJ by the scientific community continues to increase, the aged libraries it depends on constrains its development.

This project will refactor the ImageJ NanoJ plugin framework, including SRRF, into an ecosystem of napari plugins to enable next-generation high-performance, super-resolution microscopy analysis.

Technology explored

NanoJ

137 14 14 3

NanoJ-eSRRF

30 18 18 1

NanoJ-SQUIRREL

290

NanoJ-SRRF

451 20 20 2

NanoPyx

2 56 56 6