Research Team


Siân Culley

Siân Culley

Postdoc (now PI)


Started in: January 2014

Ended in: September 2021

Funding: Wellcome Trust Grant


Siân was a 8-year Postdoc (now PI) in our research group. Worked on 17 publications. Contributed to 7 technologies. Collaborated with 13 labs.

My research centres on developing novel analytical approaches for fluorescence microscopy. I helped develop the Super-Resolution Radial Fluctuations (SRRF) algorithm, which extracts super-resolution information from conventional fluorescence microscopy time-series, and allows for live-cell super-resolution imaging in physiological conditions without specialised fluorophores. I also developed the Super-Resolution Quantitative Image Rating and Reporting of Error Locations (SQUIRREL) algorithm, which is a method for assessing the quality of super-resolution microscopy images. I also develop analytical methods for microbiology, including the creation of image analysis pipelines for characterising nuclear envelope architecture during mitosis in S. pombe (in collaboration with Gautam Dey, LMCB) and characterising cell division in thermophilic Archaea (in collaboration with Uwe Schmidt, MPI-CBG).


In the news


Collaborations

Anđela Šarić

Anđela Šarić

IST
Vienna, Austria

Joint: 2

Bassam Hajj

Bassam Hajj

Institut Curie
Paris, France

Joint: 1 1

Buzz Baum

Buzz Baum

MRC-LMB
Cambridge, UK

Joint: 6 3 1

Caron Jacobs

Caron Jacobs

University of Cape Town
Cape Town, South Africa

Joint: 3 2

Christophe Leterrier

Christophe Leterrier

Aix Marseille Université, CNRS, INP
Marseille, France

Joint: 10

Dylan Owen

Dylan Owen

University of Birmingham
Birmingham, UK

Joint: 1 1

Florian Jug

Florian Jug

Human Technopole
Milan, Italy

Joint: 3 1

Guillaume Jacquemet

Guillaume Jacquemet

Abo Akademi University
Turku, Finland

Joint: 16 1

Jason Mercer

Jason Mercer

University of Birmingham
Birmingham, UK

Joint: 6 4

Loïc Royer

Loïc Royer

Chan Zuckerberg Biohub
San Francisco, USA

Joint: 2

Mark Marsh

Mark Marsh

UCL
London, UK

Joint: 4 2

Pedro Matos Pereira

Pedro Matos Pereira

ITQB NOVA
Oeiras, Portugal

Joint: 20 3

Serge Mostowy

Serge Mostowy

School of Hygiene & Tropical Medicine
London, UK

Joint: 2


Technologies

Infection-Counter Infection-Counter
NanoJ-eSRRF NanoJ-eSRRF
NanoJ-SQUIRREL NanoJ-SQUIRREL
NanoJ-SRRF NanoJ-SRRF
Sulfoscope Sulfoscope

Publications with our group (see more on Google Scholar)

High-fidelity 3D live-cell nanoscopy through data-driven enhanced super-resolution radial fluctuation
Romain F. Laine, Hannah S. Heil, Simao Coelho, Jonathon Nixon-Abell, Angélique Jimenez, Theresa Wiesner, Damián Martínez, Tommaso Galgani, Louise Régnier, Aki Stubb, Gautier Follain, Samantha Webster, Jesse Goyette, Aurelien Dauphin, Audrey Salles, Siân Culley, Guillaume Jacquemet, Bassam Hajj, Christophe Leterrier, Ricardo Henriques
Paper published in Nature Methods, November 2023
Technologies: CARE, NanoJ, NanoJ-eSRRF, NanoJ-SQUIRREL, NanoJ-SRRF, NanoPyx and Nuclear-Pores as references
Funded by: CZI, EMBO, ERC, FCT, H2021, H2022, InnOValley and Wellcome Trust
News: Photonics.com, The Science Times, Optics.org and Phys.org
Blogs: Springer Nature Protocols and Methods Community
DOI: 10.1038/s41592-023-02057-w
Physical mechanisms of ESCRT-III–driven cell division
Lena Harker-Kirschneck, Anne E. Hafner, Tina Yao, Christian Vanhille-Campos, Xiuyun Jiang, Andre Pulschen, Fredrik Hurtig, Dawid Hryniuk, Siân Culley, Ricardo Henriques, Buzz Baum, Anđela Šarić
Paper published in Proceedings of the National Academy of Sciences, January 2022
Funded by: Wellcome Trust
DOI: 10.1073/pnas.2107763119
Closed mitosis requires local disassembly of the nuclear envelope
Gautam Dey, Siân Culley, Scott Curran, Uwe Schmidt, Ricardo Henriques, Wanda Kukulski, Buzz Baum
Paper published in Nature, August 2020
Technologies: CARE, NanoJ, NanoJ-SRRF and Nuclear-Pores as references
Funded by: BBSRC and Wellcome Trust
News: Nature Asia
DOI: 10.1038/s41586-020-2648-3
The proteasome controls ESCRT-III–mediated cell division in an archaeon
Gabriel Tarrason Risa, Fredrik Hurtig, Sian Bray, Anne E. Hafner, Lena Harker-Kirschneck, Peter Faull, Colin Davis, Dimitra Papatziamou, Delyan R. Mutavchiev, Catherine Fan, Leticia Meneguello, Andre Arashiro Pulschen, Gautam Dey, Siân Culley, Mairi Kilkenny, Diorge P. Souza, Luca Pellegrini, Robertus A. M. de Bruin, Ricardo Henriques, Ambrosius P. Snijders, Anđela Šarić, Ann-Christin Lindås, Nicholas P. Robinson, Buzz Baum
Paper published in Science, August 2020
Technologies: NanoJ and NanoJ-SRRF
News: NRK, Scitech Daily, NCYT - Noticias de la Ciencia y la Technologia and UPI.com
DOI: 10.1126/science.aaz2532
Between life and death - strategies to reduce phototoxicity in super-resolution microscopy
Kalina L Tosheva, Yue Yuan, Pedro Matos Pereira, Siân Culley, Ricardo Henriques
Review published in Journal of Physics D - Applied Physics, January 2020
Technologies: CARE, NanoJ, NanoJ-Fluidics and NanoJ-SRRF
Funded by: BBSRC and Wellcome Trust
DOI: 10.1088/1361-6463/ab6b95
An Introduction to Live-Cell Super-Resolution Imaging
Siân Culley, Pedro Matos Pereira, Romain F Laine, Ricardo Henriques
Book chapter published in Imaging from Cells to Animals In Vivo, January 2020
Technologies: CARE, NanoJ, NanoJ-Fluidics, NanoJ-SQUIRREL, NanoJ-SRRF and QuickPALM
DOI: 10.1201/9781315174662-4
Live imaging of a hyperthermophilic archaeon reveals distinct roles for two ESCRT-III homologs in ensuring a robust and symmetric division
Andre Arashiro Pulschen, Delyan R Mutavchiev, Siân Culley, Kim Nadine Sebastian, Jacques Roubinet, Marc Roubinet, Gabriel Tarrason Risa, Marleen van Wolferen, Chantal Roubinet, Uwe Schmidt, Gautam Dey, Sonja-Verena Albers, Ricardo Henriques, Buzz Baum
Paper published in Current Biology, January 2020
Technologies: Sulfoscope
Funded by: Wellcome Trust
DOI: 10.1016/j.cub.2020.05.021
NanoJ - a high-performance open-source super-resolution microscopy toolbox
Romain F Laine, Kalina L Tosheva, Nils Gustafsson, Robert D M Gray, Pedro Almada, David Albrecht, Gabriel T Risa, Fredrik Hurtig, Ann-Christin Lindås, Buzz Baum, Jason Mercer, Christophe Leterrier, Pedro M Pereira, Siân Culley, Ricardo Henriques
Paper published in Journal of Physics D - Applied Physics, January 2019
Technologies: NanoJ, NanoJ-Fluidics, NanoJ-SQUIRREL, NanoJ-SRRF, NanoJ-VirusMapper and QuickPALM
Funded by: BBSRC and Wellcome Trust
DOI: 10.1088/1361-6463/ab0261
Fix your membrane receptor imaging - actin cytoskeleton and CD4 membrane organization disruption by chemical fixation
Pedro M Pereira, David Albrecht, Siân Culley, Caron Jacobs, Mark Marsh, Jason Mercer, Ricardo Henriques
Published in Frontiers in immunology, January 2019
Technologies: CARE, NanoJ, NanoJ-Fluidics and NanoJ-SQUIRREL
Funded by: BBSRC and Wellcome Trust
DOI: 10.3389/fimmu.2019.00675
Content-aware image restoration - pushing the limits of fluorescence microscopy
Martin Weigert, Uwe Schmidt, Tobias Boothe, Andreas Müller, Alexandr Dibrov, Akanksha Jain, Benjamin Wilhelm, Deborah Schmidt, Coleman Broaddus, Siân Culley, Mauricio Rocha-Martins, Fabián Segovia-Miranda, Caren Norden, Ricardo Henriques, Marino Zerial, Michele Solimena, Jochen Rink, Pavel Tomancak, Loic Royer, Florian Jug, Eugene W. Myers
Paper published in Nature Methods, November 2018
Technologies: CARE, NanoJ-SQUIRREL and NanoJ-SRRF
Funded by: BBSRC and Wellcome Trust
News: Technology Networks, VBIO, Innovations Report and Informationsdienst Wissenschaft
DOI: 10.1038/s41592-018-0216-7
Heterogeneous localisation of membrane proteins in Staphylococcus aureus
Felix Weihs, Katarzyna Wacnik, Robert D. Turner, Siân Culley, Ricardo Henriques, Simon J. Foster
Paper published in Scientific Reports, February 2018
Technologies: NanoJ and NanoJ-SRRF
Funded by: BBSRC and Wellcome Trust
DOI: 10.1038/s41598-018-21750-x
Quantitative mapping and minimization of super-resolution optical imaging artifacts
Siân Culley, David Albrecht, Caron Jacobs, Pedro Matos Pereira, Christophe Leterrier, Jason Mercer, Ricardo Henriques
Paper published in Nature Methods, February 2018
Technologies: NanoJ, NanoJ-SQUIRREL, NanoJ-SRRF and QuickPALM
Funded by: BBSRC and Wellcome Trust
News: physicsworld.com
DOI: 10.1038/nmeth.4605
SRRF - Universal live-cell super-resolution microscopy
Siân Culley, Kalina L Tosheva, Pedro Matos Pereira, Ricardo Henriques
Paper published in The international journal of biochemistry & cell biology, January 2018
Technologies: NanoJ, NanoJ-SQUIRREL and NanoJ-SRRF
Funded by: BBSRC and Wellcome Trust
DOI: 10.1016/j.biocel.2018.05.014
Fast live-cell conventional fluorophore nanoscopy with ImageJ through super-resolution radial fluctuations
Nils Gustafsson, Siân Culley, George Ashdown, Dylan M. Owen, Pedro Matos Pereira, Ricardo Henriques
Paper published in Nature Communications, August 2016
Technologies: QuickPALM
Funded by: BBSRC
News: Azom.com
DOI: 10.1038/ncomms12471
Infection Counter - Automated Quantification of in Vitro Virus Replication by Fluorescence Microscopy
Siân Culley, Greg Towers, David Selwood, Ricardo Henriques, Joe Grove
Paper published in Viruses, July 2016
Technologies: Infection-Counter
Funded by: BBSRC
DOI: 10.3390/v8070201
Mitochondria mediate septin cage assembly to promote autophagy of Shigella
Andrea Sirianni, Sina Krokowski, Damián Lobato‐Márquez, Stephen Buranyi, Julia Pfanzelter, Dieter Galea, Alexandra Willis, Siân Culley, Ricardo Henriques, Gerald Larrouy‐Maumus, Michael Hollinshead, Vanessa Sancho‐Shimizu, Michael Way, Serge Mostowy
Paper published in EMBO reports, January 2016
Funded by: BBSRC
DOI: 10.15252/embr.201541832
PALM and STORM - Into large fields and high-throughput microscopy with sCMOS detectors
Pedro Almada, Siân Culley, Ricardo Henriques
Review published in Methods, January 2015
Technologies: QuickPALM
DOI: 10.1016/j.ymeth.2015.06.004